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SRX20982711: WGS sequence of Brevibacterium sp. strain HSC-44F06
1 ILLUMINA (Illumina NovaSeq 6000) run: 533,931 spots, 254.5M bases, 83.7Mb downloads

Design: Genomic DNA libraries were prepared using the Nextera XT Library Prep Kit (Illumina, San Diego, USA) following the manufacturers protocol with the following modifications: input DNA was increased 2-fold, and PCR elongation time was increased to 45s. DNA quantification and library preparation were carried out on a Hamilton Microlab STAR automated liquid handling system (Hamilton Bonaduz AG, Switzerland). Pooled libraries were quantified using the Kapa Biosystems Library Quantification Kit for Illumina. Libraries were sequenced using an Illumina sequencer (NovaSeq 6000) using a 250bp paired end protocol. Reads were adapter trimmed using Trimmomatic 0.30 with a sliding window quality cutoff of Q15. Quality was assessed using in-house scripts combined with Samtools, BedTools and bwa-mem.
Submitted by: UC Davis
Study: Novel bacteria collected by undergraduate students
show Abstracthide Abstract
Three bacterial strains collected by Sierra College undergraduate students were tentatively identified as novel species based on 16S rDNA nucleotide sequencing. Morphological features and the results of enzymatic tests were used to verify these isolates as members of the genera Pontibacter, Hymenobacter and Planococcus or Planomicrobium. Additional information obtained through WGS sequencing and analyses of these data will be used to further characterize these isolates and determine their phylogenetic relationships with other species.
Sample: HSC-44F06
SAMN36399102 • SRS18257992 • All experiments • All runs
Library:
Name: 252700
Instrument: Illumina NovaSeq 6000
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 533,931 spots, 254.5M bases, 83.7Mb
Run# of Spots# of BasesSizePublished
SRR25236340533,931254.5M83.7Mb2023-07-11

ID:
28416543

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